As the reaction proceeds, however, reaction components are consumed, and ultimately one or more of the components becomes limiting.
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In the one-step method, RT and qPCR are performed in the same tube.
Two methods are available for quantification of gene expression by RT-qPCR: two-step RT-qPCR and one-step RT-qPCR.
Information of dependent children including name, nationality, date of birth, and passport details need to be provided.
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